https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:51036 Wed 16 Aug 2023 10:16:23 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:27033 Xenopus and is expressed during ovarian development in Drosophila. In mammals Musashi is important for spermatogenesis and male fertility, but its role in the ovary has yet to be characterized. In this study we determined the expression of mammalian Musashi proteins MSI1 and MSI2 during mouse folliculogenesis, and through the use of a MSI2-specific knockout mouse model we identified that MSI2 is essential for normal follicle development. Time-course characterization of MSI1 and MSI2 revealed distinct differences in steady-state mRNA levels and protein expression/localization at important developmental time-points during folliculogenesis. Using a gene-trap mouse model that inactivates Msi2, we observed a significant decrease in ovarian mass, and change in follicle-stage composition due to developmental blocking of antral stage follicles and pre-antral follicle loss through atresia. We also confirmed that hormonally stimulated Msi2-deficient mice produce significantly fewer MII oocytes (60.9% less than controls, p < 0.05). Furthermore, the majority of these oocytes are of poor viability (62.2% non-viable/apoptotic, p < 0.05), which causes a reduction in female fertility evidenced by decreased litter size in Msi2-deficient animals (33.1% reduction to controls, p < 0.05). Our findings indicate that MSI1 and MSI2 display distinct expression profiles during mammalian folliculogenesis and that MSI2 is required for pre-antral follicle development.]]> Wed 11 Apr 2018 16:39:10 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15689 Wed 11 Apr 2018 13:43:52 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:7798 Wed 11 Apr 2018 13:37:13 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15105 Wed 11 Apr 2018 12:24:43 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:27738 Tue 26 Feb 2019 13:24:45 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:19748 Thu 13 Aug 2015 09:19:17 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:1172 Sat 24 Mar 2018 08:28:29 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:11354 Sat 24 Mar 2018 08:08:14 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:20640 Sat 24 Mar 2018 07:55:44 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:27749 Drosophila and also shown to be vital to sperm development and reproductive potential in the mouse. We focus in depth on the role and function of the vertebrate Musashi ortholog Musashi-1 (MSI1). Through detailed expression studies and utilizing our novel transgenic Msi1 testis-specific overexpression model, we have identified 2 unique RNA-binding targets of MSI1 in spermatogonia, Msi2 and Erh, and have demonstrated a role for MSI1 in translational regulation. We have also provided evidence to suggest that nuclear import protein, IPO5, facilitates the nuclear translocation of MSI1 to the transcriptionally silenced XY chromatin domain in meiotic pachytene spermatocytes, resulting in the release of MSI1 RNA-binding targets. This firmly establishes MSI1 as a master regulator of posttranscriptional control during early spermatogenesis and highlights the significance of the subcellular localization of RNA binding proteins in relation to their function.]]> Sat 24 Mar 2018 07:27:45 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:3443 Sat 24 Mar 2018 07:20:29 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36766 Fri 03 Jul 2020 14:13:41 AEST ]]>